Using tilapia skin (Oreochromis niloticus) as an occlusive biological curative in equine wounds: short communication / Uso da pele de tilápia (Oreochromis niloticus) como curativo biológico oclusivo em feridas de equinos: nota prévia

Tilapia skin is being already use in humans and wild animals present burning wounds and showed a great result. The objective is to evaluate if tilapia skin used as an occlusive curative improves equine wound healing in two horses present chronic wound. Both animals are males, adults, both of breed Mangalarga Marchador South America. Every seven days wound we measured, photographed, biopsied for histopathological analysis, cleaned and tilapia curative was changed. Image J software was used to measure wound area. Tilapia skin as an occlusive biological factor seemed to improve healing process, wounds present an area reduction and clinical improvement during 35 days treatment, even though is still waiting for complete wound healing. In equine tilapia skin curative seemed to speed up healing process and allowed reduced curative change from every two days to once a week. This implies in decrease animal ́s stress, less pain and treatment cost reduction since we used less bandage amount. Beside that tilapia skin industrial waste. Furthermore, it allowed avoid using antibiotics, which reduces environment pollution and there ́s no antibiotic resistance issues.(AU)

A pele de tilápia está sendo utilizada em humanos e animais silvestres com feridas por queimadura demonstrando um excelente resultado. O objetivo do estudo é avaliar se a pele de tilápia utilizada como curativo oclusivo melhora o processo de cicatrização em dois equinos machos adultos da raça Mangalarga Machador, os quais apresentam feridas crônicas. A cada 7 dias as feridas eram medidas, fotografadas, biopsiadas para a análise histopatológica, limpas e o curativo de pele de tilápia trocado. O programa Image J foi utilizado para calcular a área da ferida. A pele de tilápia utilizada como curativo oclusivo parece ter um efeito positivo na cicatrização das feridas, a área diminuiu e a o aspecto clínico melhorou nos 35 dias avaliados, no entanto é necessário esperar pela cicatrização completa das feridas. Em equinos, a utilização de curativo de pele de tilápia parece diminuir o tempo de cicatrização e permite a redução do número de trocas de curativos de a cada dois dias para uma vez por semana. Isso implica em menos estresse e dor para o animal devido à menor manipulação e menor custo de tratamento, pois há menor quantidade de material de curativo sendo utilizado. Além disso, permite evitar a utilização de antibióticos, o que diminui o impacto ambiental e não gera resistência.(AU)

Apósitos de polímeros naturales para regeneración de piel / Natural polymers aposites for skin regeneration

Resumen El presente artículo es una amplia revisión bibliográfica de algunos de los polímeros naturales más utilizados en la fabricación de apósitos para curación de heridas cutáneas y regeneración de piel en los últimos años. Asimismo, se presenta un análisis descriptivo de los polímeros de origen natural más estudiados en la ingeniería de tejidos, remarcando sus propiedades físicas, químicas y biológicas. Encontrando que las investigaciones más recientes se han centrado en la exploración de apósitos a partir de biopolímeros como una alternativa a los materiales sintéticos derivados del petróleo, debido a las propiedades que poseen, como una mayor biodegradabilidad, biocompatibilidad y sostenibilidad por ser obtenidos naturalmente. Sin embargo, aún no existe un apósito ideal que pueda ser aplicado de manera eficiente en todos los tipos de heridas; por lo que los investigadores se han enfocado en el desarrollo y la optimización de apósitos que satisfagan la mayoría de las necesidades para una etapa en particular de la herida.

Abstract This review includes the bio-polymers most used in recent years for the manufacturing of materials used as dressings in cutaneous wound healing and skin regeneration. Also, the natural polymers most studied in tissue engineering are mentioned, highlighting their physical, chemical and biological properties for skin regeneration and wound healing. Through an extensive review of the clinical and research uses different types of natural polymers were compared, as well as the results of chemical and biological tests carried out during experimental research performed internationally.

Hyaluronic acid production by Streptococcus iniae and its application in rabbit skin's regeneration / 生物工程学报

Hyaluronic acid (HA) is an important biomaterial as the extracellular matrix in human body. We produced HA by fermentation of Streptococcus iniae (Strep.). Production of HA by Strep. was evaluated and further improved by strain mutation by ultraviolet. One strain with higher HA yield and lower content of protein was obtained. Its HA yield increased from (82.3±3.3) mg/L to (120±10.6) mg/L, and protein decreased from (0.178±0.011) mg/L to (0.032±0.017) mg/L. The molecular weight (MW) of HA yield from Strep. is about 3.0×10⁵ Da. Using the method of freezing and thawing, HA aqueous solution was transferred into hydrogel. This HA hydrogel, casted on sterilized non-woven fabric, was applied to repair rabbit skin with full-thickness defect. The preliminary results of the animal tests displayed that HA hydrogel obviously reduced the inflammation around the wound and promoted the skin regeneration comparing with the control tests.

Bioestimulación facial con plasma rico en plaquetas / Facial biostimulation with platelet-rich plasma

Fundamento: la bioestimulación facial con plasma rico en plaquetas es un método actual de rejuvenecimiento facial que entra dentro de las terapias regenerativas. Objetivo: mostrar el papel principal de la bioestimulación como método positivo en las características de la piel facial. Métodos: se realizó una revisión bibliográfica de un total de 140 artículos originales publicados en Pubmed, Medline, Scielo y Google académico en los idiomas inglés y español mediante el gestor de referencias bibliográficas Endnote x9, de ellos se utilizaron 48 citas seleccionadas para realizar la revisión. Desarrollo: el envejecimiento es un proceso muy complejo, donde el envejecimiento cutáneo es una de sus manifestaciones más evidentes, por lo que el cirujano plástico busca constantemente formas de lograr atenuar este proceso, una de estas alternativas es la infiltración facial con plasma rico en plaquetas. Se describe la técnica de procesamiento de la sangre para obtener el plasma rico en plaquetas y el método empleado para la bioestimulación facial. Indicaciones y contraindicaciones del proceder. Conclusiones: la bioestimulación facial con plasma rico en plaquetas es un método sencillo, libre de complicaciones dado que se trabaja con material autólogo, mediante el cual se logran cambios positivos en la piel.

Background: facial biostimulation with platelet-rich plasma is a modern method for facial rejuvenation that is part of the regeneration therapies. Objective: to show the main role of biostimulation as a positive method for the characteristics of facial skin. Methods: a bibliographic review of 140 original articles published in Pubmed, Medline, Scielo and academic Google in English and Spanish, was made by means of the reference management software Endnote x9. Forty-eight citations were selected to make the review. Development: aging is a complex process and skin aging is one of its clearest manifestations; that’s why, plastic surgeons are constantly looking for new ways to reduce this process. One of these alternatives is facial infiltration with platelet-rich plasma. The technique of processing blood to obtain platelets-rich plasma and the method used for facial biostimulation are described. Indications and contraindications of the procedure are also described. Conclusions: facial biostimulation with platelet-rich plasma is a simple, complications-free method since the procedure is made with an autologous by means of which positive changes in the skin are achieved.

Effect of Sanbai granules on patients with leucoderma / 中国生化药物杂志

Objective To investigate the effect of sanbai granules on the patients with leucoderma.Methods 180 cases with leucoderma were selected and divided into two groups.90 cases in the control group were treated by Methoxsalen Tablets 10mg,1 times a day,2h sunlight after the medication,appropriate amount external of Compound Kaliziran tincture and Halometasone.90 cases in the experiment group were treated on the base of the control group with Sanbai granules.The activity of tyrosinase,amount of melanin in the serum,clinical effect and amount of skin regeneration between two groups were compared.Results Compared with the control group, the activity of tyrosinase and melanin in serum of the experiment group were higher (P<0.05),the clinical effect rate were higher(P<0.05),number of skin regeneration piece was higher(P<0.05).Conclusion Sanbai granules can effectively improve the activity of tyrosinase and melanin in patients with leucoderma, and it has important significance for the treatment of leucoderma.

Células madre adultas como opción terapéutica para pacientes adultos con quemaduras que comprometen más del 50 por ciento de la superficie corporal total / Adult stem cells as a therapeutic option in a massive burn patient adult population with compromise of more than 50% of the body surface area

Determinar los beneficios de la terapia celular con células madre adultas como tratamiento para pacientes con pérdidas extensas de piel debido a quemaduras que comprometen más del 50% de la superficie corporal, en comparación con los beneficios del injerto autólogo de piel. Materiales y métodos. Para esta revisión de tema, se hizo una búsqueda exhaustiva en Pubmed y otras bases de datos tales como: SpringerLink, Ovid y SciELO, entre otras, de estudios analíticos observacionales, experimentales y descriptivos, sobre la terapia con células madre para la regeneración de piel y sobre el injerto autólogo de piel, de manera independiente, para compararlos entre sí. Se seleccionaron artículos que evaluaban una muestra poblacional adulta de un rango de edad de 21 a 59 años, descartando aquellos artículos que estudiaron una población infantil. Se incluyeron estudios de todos los países, en especial Estados Unidos y China por ser líderes de investigación sobre el tema, y países de Latinoamérica como Chile y Colombia, de los últimos 10 años. Para establecer los beneficios de cada procedimiento y obtener los resultados comparativos, se tuvo en cuenta el tiempo requerido por el paciente desde el inicio de cada procedimiento hasta obtener la regeneración de la piel, el porcentaje de cumplimiento del tratamiento, el número de complicaciones y su gravedad, la disminución de la morbilidad, los costos, el número de intervenciones quirúrgicas y el resultado final de la calidad de la piel regenerada, en cuanto a elasticidad, grosor y estética. Resultados. Se obtuvo información limitada sobre la terapia celular con células madre para la regeneración de piel, 24 artículos sobre colocación del autoinjerto de piel con las características específicas de la población descritas y 107 artículos utilizados como referencia para el marco teórico sobre células madre como equivalentes cutáneos. Se obtuvo como resultado que la terapia celular con células madre es un procedimiento caro ...

To evaluate the benefits of stem cell therapy for skin regeneration and to compare it with the benefits of autologous skin grafting, in a massive burn patient population with compromise of more than 50% of the body surface área Material and methods: An exhaustive search through PubMed and other databases such as SpringerLink, Ovid and SciELO was made looking for observational analytical studies, experimental and descriptive studies about stem cell therapy for skin regeneration and autologous skin grafting independently to compare them. Articles assessing an adult population sample of an age range of 21-59 years discarding items that studied a child population. Studies of United States and China were selected to be leading research on the subject, also studies of Latin American countries such as Chile and Colombia in the last 10 years were included. The next items were taken into account to establish the benefits of each procedure and obtain comparative results: the time required by the patient from the beginning of each procedure until the skin regeneration, the percentage of compliance of each procedure, the number of complications and gravity, reduced morbidity, costs, number of surgical procedures and the end result of the quality of the regenerated skin. Results. Limited information about stem cell therapy for skin regeneration was obtained, 24 articles of autograft skin with the specific characteristics of the population described were found and 107 articles more were used as references for the theoretical framework on stem cells and skin equivalents. It was obtained as a result that stem cells therapy is an expensive and laborious process, considered excellent treatment option for massive burn patients with limitations for autologous skin grafting...

Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.</p><p><b>METHODS</b>The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds. The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated. The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.</p><p><b>RESULTS</b>The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds. Mechanical properties of KCS scaffolds could be enhanced and well adjusted by chitosan. KCS scaffolds could obviously decrease bacteria number. The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration. It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples.</p><p><b>CONCLUSION</b>The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.</p>

Gene expression of the skin and sweat glands in different development stages of embryo / 中国组织工程研究

10.3969/j.issn.2095-4344.2013.24.008

Research on the proliferative scars treated by skin regenerative method treatment / 中华中医药杂志

Objective: To observe the efficacy of skin regenerative medical technique in treating proliferative scars. Method: Select 32 patients (age16-52) with proliferative scars after burns or wound for 1-11 years,which include 25(male) and 7(female). 2 scar similar spots are chosen in each patient for self-comparison.After the experimental group uses the scar detachment, scar Pi Huizhi applies the beautiful valuable moist burn medicinal plaster gauze cover the cooperation of Chinese and Western medicine home position skin regenerative method treatment; After the control group uses the scar detachment, scar Pi Huizhi applies the petroleum jelly cover the traditional method treatment.The observation comparison curative effect, applies the Vancouver scar appraisal meter appraisal scar proliferation situation. Results: Two groups return to the scar skin which plants to survive.The experimental group regenerates the skin to be good, the cicatrization speed and the quality surpass the control group (P